Nature Protocols just published detailed methods for easy LN-521 based hESC/iPSC cultivation (Rodin et al, 2014). The authors demonstrate all procedures you need for reliable, standardized and genetically stable PSC cultures. Including critical steps for easier handling and a useful trouble shooting guide.  

  • Generate clonal stem cell culture
  • Successfully change from other matrices
  • Efficiently expand human pluripotent cells
  • Freezing and thawing with high cell survival

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