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Nature Protocols just published detailed methods for easy LN-521 based hESC/iPSC cultivation (Rodin et al, 2014).
The authors demonstrate all procedures you need for reliable, standardized and
genetically stable PSC cultures.
for easier handling and a
useful trouble shooting guide.
LEARN HOW TO:
stem cell culture
change from other matrices
human pluripotent cells
with high cell survival
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